The four bcs-recombinant proteins were detected as the expected molecular weights in both induced cultures, but their overexpression was more pronounced in B21 (DE3) relative to DH5α cell lysates, in which no difference could be detected between induced and control culture profile. Given that BC is extracellularly expressed, it can be directlyBL21(DE3) Competent E.coli: C2527: 6 x 0.2ml: Agilent SURE2 Competent Cells: Agilent : 200152: 10 x 0.10ml: NEB Stable Competent E. coli (High Efficiency) C3040: 20 x 0.05ml: XL1-Blue MRF' Supercompetent Cells: Agilent: 200230: 5 x 0.2 mL: NEB ® Stable Competent E. coli (High Efficiency) C3040: 6 x 0.2 mL: BL21(DE3)pLysS Competent Cells Rosetta\Rosetta2 are BL21 so there is no different between them for bacterial expression. for eukaryotic it butter to use Rosetta because they have rare codon.
BL21 (DE3) produced the highest amount of ADI protein, followed by Rosetta (DE3). The following activity assay showed that ADI from BL21 (DE3) and Rosetta (DE3) had the most activity.
In this work, four bacterial strains, BL21 (DE3), BL21 (DE3) pLysS, Rosetta, and Arctic Express, were chosen for parallel expression trials along with BL21 (DE3) complemented with folding chaperones DnaJ/K and GroEL/ES to compare their performance in producing soluble and active human kinases. BL21 (DE3) showed the highest level of expression in inclusion bodies followed by Rosetta-gami (DE3) and Shuffle T7. Changes of expression conditions were insufficient for soluble expression of reteplase in SHuffle T7 as a genetically engineered host for production of disulfide bonded proteins. . 360 384 121 212 331 43 326 316